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Methylene Blue 20mg x 100ct
$200.00
≥99% purity · HPLC verified · MS confirmed
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Products will arrive in a lyophilized (powder) form for maximum stability
Methylene blue (methylthioninium chloride) is a redox-active compound used clinically for over a century and FDA-approved for methemoglobinemia. In research it is studied as an alternative mitochondrial electron carrier that can bypass impaired respiratory complexes, alongside low-dose effects on memory and neuroprotection. Note its narrow effective dose window and serotonergic interactions; supplied in a 20mg, 100-count format, for research use only.
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Compound Identity
COMPOUND NAME
Methylene Blue
SYNONYMS
Methylthioninium chloride, Basic blue 9, Urolene Blue, Solent blue 8
CAS NUMBER
61-73-4
MOLECULAR FORMULA
C16H18CIN3S, C16H18N3S.Cl
MOLECULAR WEIGHT
~319.85 g/mol
STRUCTURE
Organic chloride salt
PEPTIDE SEQUENCE
N/A; Not a peptide
CHEMICAL STRUCTURE
Molecular Formula: C16H18CIN3S, C16H18N3S.Cl
Source: PubChem
Mechanism of Action
Methylene blue primarily acts as a redox agent and selective inhibitor in the nitric oxide–cyclic guanosine monophosphate (NO–cGMP) pathway by directly inhibiting soluble guanylate cyclase and nitric oxide synthase, resulting in reduced vasodilation. Additionally, it participates in mitochondrial electron transfer, supports cellular respiration, and interacts with monoamine oxidase A as an inhibitor1-4.
Biological Activity
Methylene blue exhibits potent biological activity as both a redox agent and mitochondrial enhancer, promoting increased cellular respiration and decreased oxidative stress. It has demonstrated anti-aging effects by delaying cellular senescence, enhancing skin extracellular matrix components, and stimulating wound healing. Additionally, methylene blue provides neuroprotection by supporting mitochondrial function, reducing neuronal oxidative damage, and improving cognitive performance in experimental models1-4.
Storage
- Store at room temperature, protected from light and moisture.
Drug Categories
Antidote, Antimicrobial, Antimalarial, Vasopressor adjunct, Imaging agent, Antidepressant, Photodynamic therapy sensitizer
Additional Notes
Methylene blue can cause serotonin toxicity at higher doses and precipitate hemolytic anemia in patients with G6PD deficiency. Its pronounced blue color can stain biological tissues and fluids.
Summary Table
Property
Description
CAS Number
61-73-4
Molecular Formula
C16H18CIN3S
Molecular Weight (MW)
~319.85 g/mol
Mechanism of Action
Redox agent; NO–cGMP pathway inhibitor; mitochondrial electron carrier; MAO-A inhibitor
Biological Activity
Reducing agent, vasopressor adjunct, neuroprotective, antimicrobial, photodynamic sensitizer, mitochondrial electron chain (ECT) modulator
Supplied Form
Solid dye; injectable solution; oral tablets
Purity
≥99% (HPLC)
Storage
Room temperature; protect from light and moisture
Drug Categories
Antidote, Antimalarial, Vasopressor adjunct, Antimicrobial, Diagnostic, Anti-aging compound
Additional Notes
May cause serotonin syndrome or hemolysis in predisposed individuals; causes blue discoloration in fluids/tissues
Disclaimer
For Research Use Only. Not intended for human or veterinary use. This compound is supplied solely for laboratory and R&D purposes.
Detailed Product Description
Methylene blue is a multifaceted small molecule recognized for its historic clinical utility as a redox dye and medicinal antidote, as well as for its expanding therapeutic profile in modern biomedical research. At the cellular level, methylene blue enhances mitochondrial respiration by cycling electrons and increasing the efficiency of complex IV, thereby increasing ATP production and reducing the accumulation of reactive oxygen species (ROS)1. These redox-modulating effects account for its robust anti-aging activity, including the delay of cellular senescence in human fibroblasts, improved cellular proliferation, upregulation of collagen and elastin in skin, and enhanced protection against environmental stressors and age-related decline2-3. Increasing scientific attention has focused on methylene blue’s neuroprotective properties. It mitigates neurodegeneration by supporting mitochondrial energy metabolism, reducing oxidative damage, inhibiting tau aggregation linked to Alzheimer’s disease, and promoting neurogenesis and cognitive function in experimental models. Clinical and preclinical research further support its use in attenuating brain injury, enhancing wound healing, and improving age-associated cognitive performance. Methylene blue thus emerges as a unique compound bridging antidote, anti-aging agent, and neuroprotective therapeutic, with applications ranging from dermatology and regenerative medicine to neuroscience1-3.
Research Highlights
Anti-aging and Skin Longevity
: Research demonstrates that methylene blue delays cellular senescence, stimulates fibroblast proliferation, reduces oxidative stress, upregulates Nrf2 and antioxidant genes, improves skin hydration and thickness, increases elastin and collagen production, and enhances wound healing, supporting its use as a promising anti-aging agent for skin care1.
Neuroprotective Effects:
Acts as a mitochondrial electron carrier, supports complex IV activity, reduces oxidative damage, promotes neurogenesis, inhibits tau aggregation, and improves cognitive function; these properties are under investigation for prevention and treatment of Alzheimer’s, Parkinson’s, stroke, and traumatic brain injury2,4.
Mitochondrial Function and Cellular Senescence:
Methylene blue enhances mitochondrial respiration, increases oxygen consumption, boosts heme synthesis, and upregulates mitochondrial antioxidant enzymes, thereby delaying senescence in human cells and mitigating age-related mitochondrial decline3.
Treatment of Cardiovascular Collapse and Shock:
Featured as an adjunct for distributive shock in septic, anaphylactic, and drug-induced scenarios, methylene blue increases blood pressure, diminishes vasopressor requirements, and improves hemodynamic stability in refractory cases1.
NO-cGMP Pathway Inhibition
- Inhibits soluble guanylate cyclase, reducing cGMP production and reversing refractory vasodilation in shock (sepsis, anaphylaxis, drug-induced)1-3.
- Suppresses nitric oxide synthase activity, limiting vasodilatory cascades, and stabilizes blood pressure by increasing systemic vascular resistance2.
Mitochondrial Electron Transport Chain (ETC) Modulation
- Acts as an alternative electron carrier in the mitochondrial ETC, accepting electrons from NADH at complex I and directly transferring them to cytochrome c, effectively bypassing blockages in complexes I and III. This process increases the activity of complex IV, enhances ATP production, and significantly reduces the generation of reactive oxygen species (ROS) by preventing electron leakage, thereby supporting cellular energy metabolism and limiting oxidative damage2.
Monoamine Oxidase A (MAO-A) Inhibition
- Inhibits MAO-A, raising levels of serotonergic neurotransmitters; clinically relevant for potential drug interactions and risk of serotonin toxicity3.
Photodynamic Activation
- Upon NIR light exposure, acts as a photosensitizer, generating cytotoxic reactive oxygen species (ROS) that kill microbial and tumor cells3,4.
Pharmacokinetic Profile
Route of Administration
Oral
Half-Life
5-20 hours
- Route of Administration: oral, topical
- Dosing Frequency: Varies by indication and route
- Half-Life: ~5-20 hours (route dependent, shorter for intravenous)
Formulation & Handling
- Store in tightly sealed containers, protected from light
- Use only for authorized research or laboratory applications
Clinical Trial Activity
Trial ID
Title
Phase
Study Type
Sponsor
NCT02303886
Methylene Blue Intravenously and Chronic Neuropathic Pain
N/A
Interventional
Uppsala University
NCT03469284
MOM’s PAIN (Methylene Blue for Oral Mucositis’ PAIN)
2
Interventional
M.D. Anderson Cancer Center
NCT03395223
MEthylene Blue In Patients With Acquired Methemoglobinemia (MEBIPAM)
4
Interventional
Provepharm SAS
NCT06887036
Methylene Blue Treatment of Chronic Hepatitis B Virus Infection (MB_HBV)
2
Interventional
Andreas Cerny
References
1
Lo, J. C. Y., Pickering, G. & Chang, A. A Review of Methylene Blue Treatment for Cardiovascular Collapse. J. Med. Toxicol. 9, 671–677 (2013).
2
Atamna, H. et al. Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways. FASEB J. 22, 703–712 (2008).
3
Tucker, D., Lu, Y. & Zhang, Q. From Mitochondrial Function to Neuroprotection—an Emerging Role for Methylene Blue. Mol. Neurobiol. 55, 5137–5153 (2018).
4
Xiong, Z. M. et al. Anti-Aging Potentials of Methylene Blue for Human Skin Longevity. Sci. Rep. 7, 2475 (2017).
Quality & Purity
This product is synthesized via solid-phase peptide synthesis (SPPS) and tested to ≥99% purity by HPLC with identity confirmed by mass spectrometry. A Certificate of Analysis (COA) is available for every batch. Learn about our full quality and testing process →
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