Product Usage: This PRODUCT IS INTENDED AS A RESEARCH CHEMICAL ONLY. This designation allows the use of research chemicals strictly for in vitro testing and laboratory experimentation only. All product information available on this website is for educational purposes only. Bodily introduction of any kind into humans or animals is strictly forbidden by law. This product should only be handled by licensed, qualified professionals. This product is not a drug, food, or cosmetic and may not be misbranded, misused or mislabeled as a drug, food or cosmetic.

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Testagen 20mg

Name: Testagen 20mg
Brand: Grey Research Peptides
SKU: TESTAGEN20MG
Price: 70.00 USD
Availability: InStock

$70.00

≥99% purity · HPLC verified · MS confirmed How we test →

Products will arrive in a lyophilized (powder) form for maximum stability

Testagen is a synthetic tetrapeptide (Lys-Glu-Asp-Gly) developed at the St. Petersburg Institute of Bioregulation, part of the family of short peptide bioregulators studied for tissue-specific gene expression in testicular and prostate research models. Western replication remains limited. Supplied as a 20mg lyophilized vial at ≥99% HPLC purity, for research use only.

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COMPOUND NAME

Testagen

SYNONYMS

KEDG

CAS NUMBER

N/A; Not registered

MOLECULAR FORMULA

C₁₇H₂₉N₅O₉

MOLECULAR WEIGHT

~ 447.44 g/mol

STRUCTURE

Synthetic tetrapeptide

PEPTIDE SEQUENCE

H-Lys-Glu-Asp-Gly-OH

CHEMICAL STRUCTURE

Molecular Formula: C₁₇H₂₉N₅O₉

Source: PubChem

Testagen exerts its regulatory effects by penetrating cells and binding directly to DNA promoter regions, particularly CAG- or CG-rich sequences. This site-specific interaction prevents DNA methyltransferase activity, ensuring continued expression of genes critical for protein and hormone synthesis in the testes. At the cellular level, Testagen stimulates biosynthesis of cytoskeletal (actin, vimentin) and nuclear (lamin A/C) proteins that support Leydig cell structure and function, maintains chromatin in a transcriptionally active state, and suppresses apoptosis in steroidogenic cell populations^1-4.

In preclinical models, Testagen promotes proliferation and function of testicular cells, supports differentiation of stem/progenitor cells into reproductive and endocrine tissue phenotypes, and enhances protein synthesis in organs undergoing age-related regression, including the testes^1,2,4. It has been shown to improve the morphofunctional state of endocrine tissues, potentially contributing to sustained hormone output. While not a direct testosterone analog, its epigenetic and cellular effects may, in research contexts, help maintain favorable conditions for endogenous testosterone synthesis³.

Store lyophilized at ?20?°C; reconstituted solutions at 2–8?°C, use within 4 weeks. Avoid repeated freeze-thaw.

Peptide bioregulator; experimental geroprotector; endocrine regulator

N/A

Property

Description

CAS Number

N/A, not registered

Molecular Formula

C17H29N5O9

Molecular Weight (MW)

~ 447.44 g/mol

Mechanism of Action

DNA promoter binding; prevention of methylation; epigenetic activation of steroidogenesis-linked genes

Biological Activity

Supports testicular cell function; stimulates protein synthesis; maintains endocrine tissue structure; potential research model for testosterone support

Supplied Form

Lyophilized powder

Purity

≥99% (HPLC)

Storage

?20?°C dry; 2–8?°C after reconstitution

Drug Categories

Peptide bioregulator; geroprotector; endocrine function modulator

Additional Notes

N/A

For Research Use Only. Not intended for human or veterinary use. This compound is supplied solely for laboratory and R&D purposes.

Testagen is a tetrapeptide engineered to modulate the functional activity of the testes and associated endocrine glands. Through epigenetic modulation, it maintains transcriptional activity in key hormone synthesis pathways and enhances the cellular architecture required for effective steroidogenesis. In aging models, Testagen restores structural integrity, protein synthesis, and metabolic activity in endocrine tissues that support testosterone production. In vitro, it has been shown to upregulate the structural proteins that maintain Leydig cell viabilty and increase the accessibility of genome regions controlling steroidogenic enzymes^1,3. These research findings indicate that Testagen may be a valuable model compound for studying physiological testosterone regulation, particularly in the context of aging or functional decline^2,4.

Endocrine and Testosterone Support:

Regulates testicular function by maintaining chromatin in a transcriptionally active state, preventing promoter methylation of steroidogenesis-related genes, stimulating protein synthesis in Leydig cells, supporting cholesterol transport mechanisms, and reducing apoptosis in hormone-producing cells, thereby sustaining endogenous testosterone biosynthetic capacity in experimental models^1,3,4.

Stem Cell Differentiation and Immune Enhancement:

Promotes the differentiation of CD34? stem/progenitor cells into T lymphocytes and other immune-active phenotypes, increases proliferation of mature T-cells, and modifies cell receptor expression patterns to enhance immune adaptability; these effects may indirectly support endocrine–immune cross-talk and hormone homeostasis¹.

Epigenetic Gene Regulation:

Demonstrated to bind selectively to CAG- and CG-rich promoter sites, shielding them from DNA methyltransferase activity, maintaining unmethylated status, upregulating “silent” genes, and enabling sustained transcription of proteins and enzymes critical for reproductive function and cell longevity^1,2.

Cellular Structure and Function Preservation:

Increases cytoskeletal (actin, vimentin) and nuclear (lamin A/C) protein synthesis to reinforce cellular architecture in endocrine tissues, maintains optimal microenvironment for steroid production, and counteracts age-related involution of reproductive organs^1,2,4.

Anti-Apoptotic and Cytoprotective Action:

Suppresses mitochondrial-dependent apoptosis in hormone-producing and progenitor cell populations, promoting long-term viability of functional endocrine cells and supporting sustained anabolic and androgenic activity².

Selectively binds to promoter regions of genes coding for key steroidogenic enzymes (e.g., StAR, CYP11A1)^1,3.
Prevents methylation-induced silencing, enabling persistent transcription and hormone synthesis^2,4.
May indirectly enhance testosterone output under research conditions by sustaining enzyme expression^1,2.

Increases actin, vimentin, and lamin A/C synthesis, maintaining Leydig cell structure and optimal hormone production environment^1,3.
Supports transport and processing of cholesterol substrates in steroidogenic cells².

Inhibits mitochondrial apoptosis pathways in hormone-producing cells¹.
Preserves functional cell populations conducive to testosterone synthesis³.

Route of Administration

Dosing Frequency

Once daily

Half-Life

5-20 hours

Route of Administration: Intravenous injection (IV), transdermal patch
Dosing Frequency: Once daily
Half-Life: ~5-20 hours (route dependent, shorter for intravenous)

Store in tightly sealed containers, protected from light
Use only for authorized research or laboratory applications

Trial ID

Title

Phase

Study Type

Sponsor

NCT02733133

Product Transference Study of Testagen™ TDS®-Testosterone (TRANSFERENCE)

Interventional

Transdermal Delivery Solutions Corp

Khavinson, V.K. et?al. Morphofunctional fundamentals for peptide regulation of aging. Biol. Bull. Rev. 1, 3–15 (2011).

Khavinson, V.K. et?al. Mechanism of biological activity of short peptides: cell penetration and epigenetic regulation of gene expression. Biol. Bull. Rev. 3, 450–467 (2013).

Khavinson, V.K. et?al. Epigenetic aspects of peptide-mediated regulation of aging. Adv. Gerontol. 25, 11–26 (2012).

Kuznik, B.I., Pateiuk, A.V., Rusaeva, N.S., Baranchugova, L.M. & Obydenko, V.I. Effects of Lys–Glu–Asp–Gly and Ala–Glu–Asp–Gly peptides on hormonal activity and thyroid morphology in hypophysectomized mature and old birds. Adv. Gerontol. 24, 93–98 (2011).

This product is synthesized via solid-phase peptide synthesis (SPPS) and tested to ≥99% purity by HPLC with identity confirmed by mass spectrometry. A Certificate of Analysis (COA) is available for every batch. Learn about our full quality and testing process →